Fosfomycin Permeation through the Outer Membrane Porin OmpF
نویسندگان
چکیده
منابع مشابه
Trimerization of an in vitro synthesized OmpF porin of Escherichia coli outer membrane.
The assembly of outer membrane proteins of Escherichia coli was examined using the OmpF porin as a model. Since this protein is made as a precursor, which is processed to a protein of Mr 37,000 before being assembled into trimers in the outer membrane, we synthesized a modified OmpF, which lacked 16 out of 22 amino acid residues from its signal sequence, in a coupled transcription-translation s...
متن کاملImmunochemical Properties of Recombinant Ompf Porin from Outer Membrane of Yersinia pseudotuberculosis
Porins from outer membranes (OM) of Gram-negative bacteria exist in intact membranes as homotrimers forming water-filled pores or channels that provide low-molecular compounds transportation. Surface localization of the porins and their structural peculiarities determine multiplicity of their functions except for the transport one. On the one hand, porins are targets for the host immune system....
متن کاملInteraction of aminoglycosides with the outer membranes and purified lipopolysaccharide and OmpF porin of Escherichia coli.
The mechanism of uptake of aminoglycosides across the outer membrane of Escherichia coli was reevaluated. Porin-deficient mutants showed no alteration in gentamicin or kanamycin susceptibility. Furthermore, the influence of kanamycin on intrinsic tryptophan fluorescence of porin OmpF (Y. Kobayashi, and T. Nakae, Eur. J. Biochem. 151:231-236, 1985) was shown to be strongly influenced by protein ...
متن کاملComputational observation of an ion permeation through a channel protein.
The ion permeation process, driven by a membrane potential through an outer membrane protein, OmpF porin of Escherichia coli, was simulated by molecular dynamics. A Na+ ion, initially placed in the solvent region at the outer side of the porin channel, moved along the electric field passing through the porin channel in a 1.3 nsec simulation; the permeation rate was consistent with the experimen...
متن کاملEscherichia coli outer membrane protein K is a porin.
Protein K is an outer membrane protein found in pathogenic encapsulated strains of Escherichia coli. We present evidence here that protein K is structurally and functionally related to the E. coli K-12 porin proteins (OmpF, OmpC, and PhoE). Protein K was found to cross-react with antibody to OmpF protein and to share 8 out of 17 peptides in common with the OmpF protein. Strains that are OmpC po...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2019
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2018.12.002